Insertion junction: CLIP2.016665_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.016665
Chromosome:chromosome 6
Location:1690221
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g262400 (1 of 1) K14676 - lysophospholipid hydrolase (NTE, NRE) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCACCACCACACACCACCACCAGCTCCACTACCACCCACCTGGCGAGAT
Internal bar code:AATCTGCCTCCCTTGTGGGGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:892
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GGCGTACGTGAGAACACTCA
Suggested primer 2:GAAGACCATCTTGGGGTGGG