Insertion junction: CLIP2.016694_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.016694
Chromosome:chromosome 12
Location:4554591
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g521400 (1 of 1) 6.3.5.11 - Cobyrinate a,c-diamide synthase (glutamine-hydrolyzing) / Cobyrinic acid a,c-diamide synthetase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGGTCCCGCCCGGCGTGGTGATGAAGCTGTCGCTGACGCCAGGGCAGCG
Internal bar code:TTATTACCTCGGCGATGTGTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:2128
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TACACCGTCAAGCAGTCCAC
Suggested primer 2:GATCCTGCGCATAGTCGACA