Insertion junction: CLIP2.016716_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.016716
Chromosome:chromosome 3
Location:4352039
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g174672 (1 of 1) 3.1.1.93 - Mycophenolic acid acyl-glucuronide esterase / Mycophenolic acid acyl-glucuronide deglucuronidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGCTGTCGCGCTGCTGCTGCGTCAGCGCCGCCCAACGCACCTCCGACAG
Internal bar code:CGATGTCGTTGGTTTCCGGAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2784
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTTCGACTACTCGGGGTA
Suggested primer 2:AGTTCCGCAACACTCGTCAT