Insertion junction: CLIP2.016738_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.016738
Chromosome:chromosome 16
Location:602822
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g692150 GT90-29,GT90F29 (1 of 11) 2.4.2.26 - Protein xylosyltransferase / Uridine diphosphoxylose-protein xylosyltransferase; GT90 family protein 29 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTCGTATATGTCGTCGATCAAGCGAGATGTGGTGTTCCAGAACGGCACG
Internal bar code:ATATTCTAACTTAGCCCTATAT

Confirmation - LEAP-Seq

LEAP-Seq distance:702
LEAP-Seq percent confirming:60.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GCAATCAGGGCAAGGTCTCT
Suggested primer 2:GAACAGGGACTTGTACCGCA