Insertion junction: CLIP2.016771_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.016771
Chromosome:chromosome 9
Location:2400135
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g393750 (1 of 1) PTHR24031:SF284 - DEAD-BOX HELICASE DBP80 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCTCTCCCTGGGGCTGCTGCTGCTGCCAACGCTGCCGGCGCTGCAACGG
Internal bar code:ACCCTATTTGTTCACAACGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:357
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AATCCAATACCCGCAGCACA
Suggested primer 2:CGCTGTCTCCGAAGCCATAT