Insertion junction: CLIP2.016776_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.016776
Chromosome:chromosome 6
Location:2805724
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g271800 LCI36 Low-CO2-inducible membrane protein; (1 of 4) K15382 - solute carrier family 50 (sugar transporter) (SLC50A, SWEET) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCGATCTGCATCACACTCAGCGCTGCACGGCGGCGGCGTTGGGGCAGT
Internal bar code:CGAGGTGCTCTATTTTCCTGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:1802
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:83
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:83

Suggested primers for confirmation by PCR

Suggested primer 1:ATGAGGTTAGCGGAGGACCT
Suggested primer 2:AAATCGCCCTCTCACTTGCA