Insertion junction: CLIP2.016796_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.016796
Chromosome:chromosome 14
Location:4126893
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g633900 (1 of 1) K01008 - selenide, water dikinase (selD, SEPHS) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAGGGAACGGTTTGACCCGGTCCTCCACCCCGATCTCCCGAGCACACA
Internal bar code:GCAATAGGAAGGGTAAATTGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:401
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CCAAACCACTCCCATACCCC
Suggested primer 2:TGCACAGGGATACACGTACG