Insertion junction: CLIP2.016818_3

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.016818
Chromosome:chromosome 17
Location:470614
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre17.g699000 PAT1 Phosphate acetyltransferase; (1 of 2) 2.3.1.8 - Phosphate acetyltransferase / Phosphotransacetylase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACTCCCAGCACGTCGACCTTGTGGTCCGCAAACACCTGCCGCTTCACC
Internal bar code:AAGAGTAGTCCTTCGAGCGGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:672
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:AACGCCACAGCCAATGACTA
Suggested primer 2:GGTTTCAGGGGACAGCGTAA