Insertion junction: CLIP2.016828_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.016828
Chromosome:chromosome 11
Location:2077085
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467756 MMP5 Metalloproteinase of VMP family; (1 of 51) PF05548 - Gametolysin peptidase M11 (Peptidase_M11) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCCCCAACACACACACACATACACACACAGGCGACGCCTACCTCATCT
Internal bar code:CGATTCAATATGTCCAGCGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:529
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TGGAATCAAAGGGGGATGGC
Suggested primer 2:TTTGAGGGTCGGCTGGTAAC