Insertion junction: CLIP2.016847_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.016847
Chromosome:chromosome 10
Location:6053711
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g801203 (1 of 100) IPR001810 - F-box domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCTCTTAAAGGTCGGCACAGCAGCTGCTGCTGAGGCGGTCGAGGGGGCA
Internal bar code:GCTCGACGACGGTCTCTAGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2717
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:CGGAGACATTGTCACAGGCT
Suggested primer 2:CATGGTGAGGAGGAGGAGGA