Insertion junction: CLIP2.016852_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.016852
Chromosome:chromosome 10
Location:768257
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g422750 MOT10 Predicted protein; (1 of 3) IPR002110//IPR005821//IPR020683 - Ankyrin repeat // Ion transport domain // Ankyrin repeat-containing domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTTGTGACGTCATCACGCTCACGTCTTGCATGGACGGCTACCCCACGTG
Internal bar code:TGTCGAGGGCGGGATCTGGAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:4193
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:55
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:55

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTCCAACAGACTGATGCG
Suggested primer 2:CGCTGCGATGTCCATTGAAG