Insertion junction: CLIP2.016882_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.016882
Chromosome:chromosome 6
Location:6417393
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g293516 DEGO2 (1 of 4) PTHR22939:SF83 - PROTEASE DO-LIKE 2, CHLOROPLASTIC; Inactive Deg protease intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGGCTGCCGCCACGGAAGCAGCCACAGCCCACGTACCCATACCCCCACC
Internal bar code:TAATGGTGATCACGCGCTATAC

Confirmation - LEAP-Seq

LEAP-Seq distance:127
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ATCTACTGCTGCTACTGCCG
Suggested primer 2:CACACACAACCTCCTCCTCC