Insertion junction: CLIP2.016926_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.016926
Chromosome:chromosome 7
Location:5874202
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g353750 MMP10 Metalloproteinase of VMP family; (1 of 40) 3.4.24.38 - Gametolysin / Lysin intron
Cre07.g800866 (1 of 51) PF05548 - Gametolysin peptidase M11 (Peptidase_M11) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCACGTGAAGGGCTGGGCAACCATGTGCGTGTGAAGCCCACATGGACGT
Internal bar code:CCAAGCTGGCAGGGTTTTCTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:3811
LEAP-Seq percent confirming:93.8462
LEAP-Seq n confirming:61
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:65

Suggested primers for confirmation by PCR

Suggested primer 1:GGACGTTGTGGTTGTCGTTG
Suggested primer 2:TCAGTCGTCTAGGTCCAGCA