Insertion junction: CLIP2.016932_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.016932
Chromosome:chromosome 17
Location:4719471
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g732901 (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCACGCACGTCCCGCACACACACCGTAGGCGCCTGCTCTTAACCGCTGCC
Internal bar code:GGTTCCGGTGCGTCTGGACGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:3774
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:51
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:51

Suggested primers for confirmation by PCR

Suggested primer 1:AAACGCACCACTGCATGTTC
Suggested primer 2:TAATCCGCCTTCGTCGTAGC