Insertion junction: CLIP2.016936_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.016936
Chromosome:chromosome 2
Location:5288745
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g112800 (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCCCCAATCCGCTGGGAACTCCCAGGTGCGCCCATGGACCCGGCGACAC
Internal bar code:AAACATACTTCATCCCAAGGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1737
LEAP-Seq percent confirming:95.4545
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:CTTGGAGCGGTCTGTGTCAT
Suggested primer 2:GAGGCTTGCTATCCCTCGTC