| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.016937 |
| Chromosome: | chromosome 12 |
| Location: | 9047568 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g544700 | PUF3 | Puf protein; (1 of 1) PTHR12537:SF13 - SUPPRESSOR PROTEIN MPT5 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTGTGGTGACCATGCGAAAGCGTGTCACAGCATGTCAAGCAAGCCGAG |
| Internal bar code: | GGCTGTCGTATTGCAACCGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3004 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTCTTTCCCGCTCTTGTC |
| Suggested primer 2: | GGGTTCACTGCAGCACAATG |