| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.016946 |
| Chromosome: | chromosome 1 |
| Location: | 3937129 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g025850 | INO | (1 of 1) 1.13.99.1 - Inositol oxygenase / Myo-inositol oxygenase; myo-inositol oxygenase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTCACCGCCAACCCCGACCGCCGCCGCCGCCTGTACAACACACAGACC |
| Internal bar code: | TCGCTTTTCTAGTAATTAGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4319 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAAACTTGGAGTGGCGCAG |
| Suggested primer 2: | GCACCTGGTGGGACTCATAC |