Insertion junction: CLIP2.016965_3

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.016965
Chromosome:chromosome 15
Location:1461103
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre15.g641850 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCCCTCGCTAAGCACAACGTTGCACACGCACCAATCCAATCCTCTAGCC
Internal bar code:ATTGCACCCACGATGACTAATT

Confirmation - LEAP-Seq

LEAP-Seq distance:2992
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:AAAAGTGTTTCCGACGCGTG
Suggested primer 2:CGCGTTGTTGTTGTTGTTGC