Insertion junction: CLIP2.016971_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.016971
Chromosome:chromosome 16
Location:4186906
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g670300 PRP39 (1 of 1) K13217 - pre-mRNA-processing factor 39 (PRPF39, PRP39); Nuclear pre-mRNA splicing factor and U1 snRNP Component intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAACTGCCACACCCGTGCTTAGCGCACAGCTCTTTGCACATGCCCTCTCC
Internal bar code:ATTTCCAATAGCGATAAAATTG

Confirmation - LEAP-Seq

LEAP-Seq distance:159
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CTTCGGCTAGGGATCGTGAC
Suggested primer 2:CTTTCTCACCCAATCCGCCT