Insertion junction: CLIP2.016972_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.016972
Chromosome:chromosome 2
Location:3053871
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g095750 ZEP1,ZXE1 (1 of 1) 1.14.13.90 - Zeaxanthin epoxidase / Zea-epoxidase; Putative zeaxanthin epoxidase-like protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACAATGCACGCTCTCACCCGGCTTGTGTGCGTCCACCACTGTGTTGCCG
Internal bar code:TGCATCTAATTCATATGTGCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:146
LEAP-Seq percent confirming:85.7143
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:ATGGGGCTAGGAGGGTCATT
Suggested primer 2:CTGTGCGTGTACTTGGACCT