| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.016975 |
| Chromosome: | chromosome 9 |
| Location: | 3333334 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g395436 | subunit 4 of splicing factor 3b (SF3b); (1 of 1) PTHR24012//PTHR24012:SF370 - FAMILY NOT NAMED // RNA-BINDING PROTEIN 44 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGGCGGCGGGAAGTGGCCTGCGCCCTCCAGCGGCGGCGTGGGCGGCGC |
| Internal bar code: | AAGGATTAGAGCCCTGCTGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1821 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGGCGATTGAGGTGAGGG |
| Suggested primer 2: | TCACCGTCATCATGACCTGC |