Insertion junction: CLIP2.017009_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.017009
Chromosome:chromosome 12
Location:9546616
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g540050 (1 of 12) IPR000595//IPR018490 - Cyclic nucleotide-binding domain // Cyclic nucleotide-binding-like intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCGTGTACAATTGGACCAGCATCGCACTATGTTTTCCGTCTGCTTCATC
Internal bar code:GTAGGGGGCATCAATTGGTTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1312
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:TGACGCTGTCCAACACTACC
Suggested primer 2:TGTGTGTGTGTGTGTGTGGA