Insertion junction: CLIP2.017016_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.017016
Chromosome:chromosome 12
Location:1234332
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g490050 TPT18,GMT1,TPT19 (1 of 3) K15356 - GDP-mannose transporter (VRG4, GONST1); Putative GDP-mannose transporter intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGCACTTCCGGGGGCTCATTCCTCGTGCCGGCTGTGCTGGGTCTCTGGT
Internal bar code:CGATGATATTTTCTGACCAGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2045
LEAP-Seq percent confirming:91.3043
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:GGCCACGAAGATGAGGTTCA
Suggested primer 2:CCTGACGCAGGTAGGTTTGT