Insertion junction: CLIP2.017042_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.017042
Chromosome:chromosome 10
Location:5876678
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g460600 CYN53 (1 of 2) PTHR11071//PTHR11071:SF297 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE // CYCLOPHILIN-LIKE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FAMILY PROTEIN; Cyclophilin 53 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGGTAGAAACCCCCACAGGCAACGGCGGGGAGTGCAGTGATGGTGCCCG
Internal bar code:CATAGCGGGAGCACAATCCGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2716
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:ACATTGATGACCGCCGAGTT
Suggested primer 2:AGCCGTTCATGTGGAGAAGG