| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.017051 |
| Chromosome: | chromosome 10 |
| Location: | 2458916 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g435550 | DOH1 | Deoxyhypusine hydroxylase/monooxygenase; (1 of 1) 1.14.99.29 - Deoxyhypusine monooxygenase / DOHH | 3'UTR |
| Cre10.g435600 | TMEM17 | Transmembrane protein 17; (1 of 1) K19384 - transmembrane protein 17 (TMEM17) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGCCGCTTCTGCTTGCGGTACATGGAGAACACCTGGGGGCGTTTTGTG |
| Internal bar code: | TGCTATTAGTGTTCCATCTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2759 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 85 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 85 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATCCATTGTGTCGCAGCT |
| Suggested primer 2: | TGGAGTAGGGGGAGACGAAG |