| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.017054 |
| Chromosome: | chromosome 3 |
| Location: | 1239543 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g149700 | (1 of 1) PF14518 - Iron-containing redox enzyme (Haem_oxygenas_2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCGCCGCTCCGTCAGCAGGAAGTTGTGGTTGGCGCCCGCCAGCCACT |
| Internal bar code: | TAGTCCTTATGTTGCGCCGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1163 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGCGTTCTGTTTGGGGTG |
| Suggested primer 2: | GCATCAGGACACAGGCAGAT |