Insertion junction: CLIP2.017064_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.017064
Chromosome:chromosome 9
Location:173501
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g386736 FAP44 Flagellar Associated Protein 44; (1 of 1) PTHR13720//PTHR13720:SF18 - WD-40 REPEAT PROTEIN // WD REPEAT-CONTAINING PROTEIN 52 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAAGAGGCAGCAATTGCAGATGAAACAGGGTCAAAGCCTCCGGCCGAGG
Internal bar code:CGAGACGTCTTCTCGTGCGGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:4704
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:35
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:ATCAGTGGTCGGCTTTCCTG
Suggested primer 2:TTGCACTCGCTAAGAAGGCA