| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.017084 |
| Chromosome: | chromosome 3 |
| Location: | 412672 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144847 | PYK4 | (1 of 2) PTHR11817//PTHR11817:SF3 - PYRUVATE KINASE // PYRUVATE KINASE; Pyruvate kinase 4 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGCACTGGCGCGCCACGTGCGTGCGCGTGGTCACCACCACAATGGCCT |
| Internal bar code: | TATTGGTCAAGGCGTTACCGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5557 |
| LEAP-Seq percent confirming: | 98.8506 |
| LEAP-Seq n confirming: | 86 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 87 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGTTGACGCGCTAAATCC |
| Suggested primer 2: | CGTGCACACCACCTTAGTCT |