| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.017095 |
| Chromosome: | chromosome 10 |
| Location: | 5134573 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g455400 | RME2 | (1 of 1) 2.1.1.223 - tRNA(1)(Val) (adenine(37)-N(6))-methyltransferase; RNA methylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCATCCACCTCTTATACCTCAACAAACGCCCCACCCCACCTGCAACACC |
| Internal bar code: | GTCAGGGCGACGGTGAGACTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1612 |
| LEAP-Seq percent confirming: | 68.75 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGACGTGTGTGTTGGTTG |
| Suggested primer 2: | GTGTGCATGCGTGTGTGAAT |