| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.017122 |
| Chromosome: | chromosome 17 |
| Location: | 5827754 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g802123 | (1 of 70) 4.6.1.1 - Adenylate cyclase / ATP pyrophosphate-lyase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCACCTACCTGCATGAACGAGGCGGTGGAGATGTCGGACGCCACGTCCG |
| Internal bar code: | GCTGGGGGTACGAAGTTGGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5167 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 44 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAACAGTGGCATCAGCAAC |
| Suggested primer 2: | TTTGGGCTCCATACGGAACC |