Insertion junction: CLIP2.017125_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.017125
Chromosome:chromosome 7
Location:3532831
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGACTCCTTTCCCCACCGCCTCCTCACCACCTACTCTCACCCTCAATCCC
Internal bar code:GTACGTATAGGGTAGATTCGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:3191
LEAP-Seq percent confirming:97.8723
LEAP-Seq n confirming:46
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:47

Suggested primers for confirmation by PCR

Suggested primer 1:CGCATCCCACGTGTTTGTTT
Suggested primer 2:CAGGTGTGAAGTACGGCAGT