| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.017125 |
| Chromosome: | chromosome 12 |
| Location: | 7361922 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g557750 | DGTT1 | Diacylglycerol O-acyltransferase, Type 2; (1 of 2) PTHR12317:SF0 - DIACYLGLYCEROL O-ACYLTRANSFERASE 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCGGAAATGGCGATGACGCCGTGCGGGTGGCTCACGAATATATAGCGG |
| Internal bar code: | GAGGTGTTCCGCAGAGGACTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3337 |
| LEAP-Seq percent confirming: | 98.3051 |
| LEAP-Seq n confirming: | 58 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACACGAGCTTGCAGCACT |
| Suggested primer 2: | TGCCGTGCGATAGGATGTAC |