| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.017128 |
| Chromosome: | chromosome 11 |
| Location: | 357717 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467576 | (1 of 1) PTHR33403//PTHR33403:SF5 - FAMILY NOT NAMED // PROTEIN SPIRAL1-LIKE 2-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATTGACATGAGGCGTGGAAAATGGTGTTGGGTTACGCGGCACAGCCGCC |
| Internal bar code: | GTTGTCGTAATAGGGTATCAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1553 |
| LEAP-Seq percent confirming: | 96.4286 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAAGCTTGTGAGGCCTCT |
| Suggested primer 2: | CACCTCCAACCAGCTCTCTG |