Insertion junction: CLIP2.017188_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.017188
Chromosome:chromosome 14
Location:1251206
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g616050 (1 of 8) PF00169 - PH domain (PH) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCTCACCAGTCGTCTCCGCTCCCCCGCTCATGCTCCCAATCCTGCGCGC
Internal bar code:AGGTTGGTTACAGGAATCGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1845
LEAP-Seq percent confirming:95.8333
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:TGTCAAAGTCGTCTGCTCCC
Suggested primer 2:CAAGGCGATGTTAATGCGGG