Insertion junction: CLIP2.017194_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.017194
Chromosome:chromosome 12
Location:2649810
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g505650 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAGAACTCGGGGGTGTGCGCTGCACCGCTGAGGTCCCCGTGAGCGTGTG
Internal bar code:TCATTCGATTCTGATATATGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:3029
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCACGCCCTAATGCATTG
Suggested primer 2:GTCATCATCAAAGTCGCCGC