| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.017238 |
| Chromosome: | chromosome 6 |
| Location: | 6539956 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g800721 | (1 of 1) K17582 - antigen KI-67 (MKI67) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGACCCAGGCTCTGGGCTGGGTGGCGCAGGGCTGGGTGGCTTCGGGCTG |
| Internal bar code: | GAGAAGTTATATGGAGCGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2698 |
| LEAP-Seq percent confirming: | 60.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGGGTCTCCTCCTCCTTC |
| Suggested primer 2: | GAAGGAGGAGGAGACCCAGG |