| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.017243 |
| Chromosome: | chromosome 6 |
| Location: | 5397818 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g285500 | (1 of 2) K13173 - arginine and glutamate-rich protein 1 (ARGLU1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTATCCCAAGGAGGCTGGATTTAGGAGCACATTCTGAGCTATCTACCGTG |
| Internal bar code: | AAGGTTCCAAATGTGCGGGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2159 |
| LEAP-Seq percent confirming: | 71.4286 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGGTGTTAGGGCGGGATG |
| Suggested primer 2: | CAGCGGTGCAAAATGGTTGA |