| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.017257 |
| Chromosome: | chromosome 10 |
| Location: | 1923562 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g431650 | (1 of 2) 2.7.1.86 - NADH kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGATGCATGTTTCTCTGACGGAAGTTGCATAAATAGTCTGGATGCCTG |
| Internal bar code: | ATGCCTCGACGTGCGCTGAACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2465 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTAGACGGTAGACCAGGG |
| Suggested primer 2: | GCTGCATTTCCGGTACACAC |