Insertion junction: CLIP2.017279_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.017279
Chromosome:chromosome 9
Location:1914162
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g397300 (1 of 7) PF02190 - ATP-dependent protease La (LON) substrate-binding domain (LON_substr_bdg) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCGCGCACAGCCACAGAGGCGTCCGTCGCAGCCGAGTCGACGGCTGCG
Internal bar code:GTCATGGTCTAGGCAGGATTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2632
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:GCCCTAACAACGTCCAGGAA
Suggested primer 2:GGCACCCCTTAACCTTACCC