Insertion junction: CLIP2.017302_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.017302
Chromosome:chromosome 1
Location:3968582
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g025983 protein of unknown function with DUF1399 domain; (1 of 11) PF07173 - Protein of unknown function (DUF1399) (DUF1399) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGGTGGGGTACTGGCCCGCCGGCCGGACGGCTGTAGCAGCGGCGGCGC
Internal bar code:GGGCTATGGCACGTACCGGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1312
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:TCCCGCACTCAACAACATCA
Suggested primer 2:GGCTCTGCGACTGACTGTAG