Insertion junction: CLIP2.017319_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.017319
Chromosome:chromosome 2
Location:233156
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g074600 DeSI-2,CGL60,DESI2 (1 of 6) PF05903 - PPPDE putative peptidase domain (Peptidase_C97); DeSI-type SUMO protease CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCTTGAGGCGTCCGCAGGCGACGGAACGAAACAGTTCCTGGTGAGGTTG
Internal bar code:CCCATTCAAGGGCTAGTGCGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2618
LEAP-Seq percent confirming:96.2264
LEAP-Seq n confirming:51
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:53

Suggested primers for confirmation by PCR

Suggested primer 1:GACCCACACTTGCCACAGTA
Suggested primer 2:CCTGTTACGCTGCCCTGTTA