Insertion junction: CLIP2.017323_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.017323
Chromosome:chromosome 3
Location:2504983
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g159400 (1 of 2) 2.7.7.73 - Sulfur carrier protein ThiS adenylyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTGGTAGTCGTAGGCCTAGGAGTAAGTTGGCTATGCTATACTTTTGTCT
Internal bar code:TTCGCTACGCGTCTTCCTTATT

Confirmation - LEAP-Seq

LEAP-Seq distance:3728
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGCCGCTGAAAGAAAAGC
Suggested primer 2:CGCGCGCTTGTGATATTTGA