| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.017325 |
| Chromosome: | chromosome 3 |
| Location: | 4099802 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g172350 | (1 of 1) PF14736 - Protein N-terminal asparagine amidohydrolase (N_Asn_amidohyd) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAAGGTACAGTATCCGTGACAATGGTGGCAGCAGCGTAGTGCATGGTTG |
| Internal bar code: | ATATAGTTATCATGAGGACAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3280 |
| LEAP-Seq percent confirming: | 98.7013 |
| LEAP-Seq n confirming: | 76 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 77 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAAGCTCGTACGGGAAGCG |
| Suggested primer 2: | CGGCTGTAGTAGAGCAGGTG |