Insertion junction: CLIP2.017330_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.017330
Chromosome:chromosome 2
Location:3227588
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g097050 (1 of 8) PF01697 - Glycosyltransferase family 92 (Glyco_transf_92) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCAAAGGGGAGGAGTTTCCGGGAGGCTTCCAGGTATGAGTGCTGGCGAA
Internal bar code:AGGGGGATGGTACACAACGTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:3356
LEAP-Seq percent confirming:93.5484
LEAP-Seq n confirming:87
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:93

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTGCACTTGTTGAAGGTG
Suggested primer 2:GGCGTCTTGGCTTTCACAAG