Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | + |
Strain: | CLIP2.017346 |
Chromosome: | chromosome 10 |
Location: | 338345 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g420400 | (1 of 1) K14289 - exportin-5 (XPO5) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCCGCCGCAACACTTCAATGTTGCCTTGCACATTGGGAAACCGAGCAA |
Internal bar code: | TTTCTTCTCTGGATACACTCGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 3038 |
LEAP-Seq percent confirming: | 92.5926 |
LEAP-Seq n confirming: | 75 |
LEAP-Seq n nonconfirming: | 6 |
LEAP-Seq n unique pos: | 81 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTTTTCGTGGAGGGGTTTGC |
Suggested primer 2: | CACAATTTCCGGCGTGTTGT |