Insertion junction: CLIP2.017363_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.017363
Chromosome:chromosome 4
Location:2365092
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g221150 GT90-5,GT90F5 GT90 family protein 5; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCTGATGAGGTGAGCGGCACCCTCGCGCGCCAGGGCTTCAGGTCTGCCT
Internal bar code:TCCATTCAGTATTTACTTTGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2448
LEAP-Seq percent confirming:92.8571
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:AAAATAGACGCTGGGTGGCA
Suggested primer 2:CTTTCAACCCTGTTGCCTGC