| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.017367 |
| Chromosome: | chromosome 14 |
| Location: | 3338473 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g629600 | (1 of 1) PF06803 - Protein of unknown function (DUF1232) (DUF1232) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCTACGTAGCGCCGTAGTGTCATACTGCACTCACGCACCCAGAGCAGC |
| Internal bar code: | GTAAGCATCAATCATGTTTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3693 |
| LEAP-Seq percent confirming: | 92.8571 |
| LEAP-Seq n confirming: | 52 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGCGCCAAGTAACAGCCA |
| Suggested primer 2: | TACGATTCGCCTGGATCTGC |