Insertion junction: CLIP2.017418_3

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.017418
Chromosome:chromosome 17
Location:1563239
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g707150 LIC2 Hypothetical protein; (1 of 45) IPR029052 - Metallo-dependent phosphatase-like CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGTAAGTCTGATAACAATGCAATCTTCATCGTCCACGCCGCGGCAAAAA
Internal bar code:TCTCAATTTTTAGAATGCACGG

Confirmation - LEAP-Seq

LEAP-Seq distance:3577
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:57
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:57

Suggested primers for confirmation by PCR

Suggested primer 1:CGCACAAGGTTATAGCGCAC
Suggested primer 2:GTAGCGCCGTTTGCATACTG