Insertion junction: CLIP2.017428_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.017428
Chromosome:chromosome 6
Location:8068404
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g305250 (1 of 90) PF03372 - Endonuclease/Exonuclease/phosphatase family (Exo_endo_phos) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATTGGAGGGCCCTCCGCTGCCATCGGCCGCAGCCCGGCTGACTGCTGAG
Internal bar code:CTTGTAGGTCTGTTCGCGTGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1284
LEAP-Seq percent confirming:52.6316
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCACACTCTTTCCCACCC
Suggested primer 2:CCATGATGCCGTTGCTGATG