| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.017482 |
| Chromosome: | chromosome 3 |
| Location: | 1280611 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g150050 | (1 of 6) 3.1.3.4 - Phosphatidate phosphatase / Phosphatidic acid phospphatase | CDS | |
| Cre03.g150101 | NRT2D | Nitrate/nitrite transporter; (1 of 5) PTHR23515//PTHR23515:SF2 - FAMILY NOT NAMED // HIGH AFFINITY NITRATE TRANSPORTER 2.3-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGAATGCTTGACTTTCTCGTCGCTGCGCCTGGCGCTTTGCTATTGCTG |
| Internal bar code: | CTTGGCTGGATACTCGACCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1457 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTAGGTGCTCACAGCCATG |
| Suggested primer 2: | AACGTGAAGCACCCACCTAG |